ABSTRACT
Berries and branches essential oil of Juniperus phoenicea were obtained by electromagnetic induction heating assisted extraction and by hydrodistillation with a yield varied from (1.2 ± 0.3 to 2.4 ± 0.7%) and from (0.6 ± 0.1% to 1.1 ± 0.1%), respectively. forty eight compounds were identified representing (97.2 – 99.7%) of the oil. α-Pinene (40.3 – 67.8%) and δ-3-carene (13.5 – 26.8%) were the main compounds in berries and branches essential oils. Antioxidant activity was evaluated by three means: inhibition of 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical, reducing power and β-Carotene/linoleic acid bleaching. The antioxidant activity of essential oils showed IC₅₀ ranging from 67.6 ± 1.02 µg/mL to 131.5 ± 0.8 µg/mL for berries and from 98 ± 1.25 µg/mL to 166.8 ± 0.29 µg/mL for the branches. Berries oil show more potent antioxidant activity compared to branches. This result is supported by the three methods investigated in this work.
Subject(s)
Fruit , Heating , Hot Temperature , Juniperus , Magnets , Oils, VolatileABSTRACT
The present study deals with the determination of optimal values of operating parameters such as the temperature of heating, the mass of the plant material and the volume of water leading to the best yield of electromagnetic induction (EMI) heating extraction of Algerian Thymus fontanesii essential oil. After an appropriate choice of the three critical variables, eight experiments leaded to a mathematical model as a first-degree polynomial presenting the response function (yield) in the relation to the operating parameters. From the retained model, we were able to calculate the average response, the different effects and their interactions. The maximum of essential oil recovery percentage relative to the initial mass of plant material was 1.69%, and was obtained at (140 ℃, 250 g and 4.5 L). The chemical composition of the Algerian T. fontanesii essential oil under the obtained optimal conditions (140 ℃, 250 g and 4.5 L), determined by GC/MS and GC/FID, reveled of the presence of major components such as: carvacrol (70.6 ± 0.1%), followed by p-cymene (8.2 ± 0.2%).